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Becton Dickinson fixable viability stain 700
Fixable Viability Stain 700, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fixable+viability+stain+700/fixable+viability+stain+780/pm40358179-122-8-12
Average 90 stars, based on 1 article reviews
fixable viability stain 700 - by Bioz Stars, 2026-11
90/100 stars

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Staining:

Article Title: ADAR1 prevents ZBP1-dependent PANoptosis via A-to-I RNA editing in developmental sevoflurane neurotoxicity
Article Snippet: .. After sevoflurane priming (3%, 6 h), hippocampal neurons were stained with Fixable Viability Stain 700 (BD, USA) and permeabilized with Cytofix/Cytoperm (BD, USA) according to the manufacture's instructions. ..

Article Title: Homologous but not heterologous COVID-19 vaccine booster elicits IgG4+ B-cells and enhanced Omicron subvariant binding
Article Snippet: .. Briefly, 10–15 million PBMC were incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD21, CD27, CD38, CD71, IgA, IgD, IgG1, IgG2, IgG3, IgG4, (Cat numbers and dilutions provided in Supplementary Tables and ) and μg/ml each of [RBD WH1] -BUV395, [RBD WH1] -BUV737, and [RBD BA.2] -BV480 and [RBD BA.5] -BV650 for 15 min at room temperature in a total volume of 250 μl FACS buffer (0.1% sodium azide, 0.2% BSA in PBS). .. In addition, 5 million PBMC were similarly incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD27 and IgD, and BUV395-, BUV737-, BV480- and BV650-conjugated streptavidin controls (Supplementary Tables and ).

Article Title: Influence of Donor-Specific Characteristics on Cytokine Responses in H3N2 Influenza A Virus Infection: New Insights from an Ex Vivo Model
Article Snippet: Molecular expressions were analyzed using the FACSAria TM IIu Cell Sorter and FACSDiva TM software v5.0.3 (Becton Dickinson Biosciences). .. An unstained singlet population from the digested tissue was used to establish a baseline, and dead cells were excluded using Fixable Viability Stain 700 (Becton Dickinson Biosciences, catalog No: 564997), enabling accurate analysis of IAV-related molecular expressions in lung tissue samples. ..

Article Title: Homologous but not heterologous COVID-19 vaccine booster elicits IgG4+ B-cells and enhanced Omicron subvariant binding
Article Snippet: Briefly, 10–15 million PBMC were incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD21, CD27, CD38, CD71, IgA, IgD, IgG1, IgG2, IgG3, IgG4, (Cat numbers and dilutions provided in Supplementary Tables and ) and μg/ml each of [RBD WH1] -BUV395, [RBD WH1] -BUV737, and [RBD BA.2] -BV480 and [RBD BA.5] -BV650 for 15 min at room temperature in a total volume of 250 μl FACS buffer (0.1% sodium azide, 0.2% BSA in PBS). .. In addition, 5 million PBMC were similarly incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD27 and IgD, and BUV395-, BUV737-, BV480- and BV650-conjugated streptavidin controls (Supplementary Tables and ). ..

Article Title: Enhanced Expression of Mitochondrial Magmas Protein in Ovarian Carcinomas: Magmas Inhibition Facilitates Antitumour Effects, Signifying a Novel Approach for Ovarian Cancer Treatment.
Article Snippet: .. The harvested cells were stained on ice with Fixable Viability Stain 700 (BD Horizon Cat 564997). .. A total of 7000 cells were analysed using a four-laser LSR Fortessa (BD Biosciences, Melbourne, Australia), with TMRM detected in the PE channel (Excitation 561 nm, Emission detector 586/15 nm).

Article Title: From foe to friend: Rewiring oncogenic pathways through artificial selenoprotein to combat immune-resistant tumor
Article Snippet: .. The cells were subsequently washed with PBS and stained with Fixable Viability Stain 700 (FVS700; Becton, Dickinson and Company, Franklin Lakes, NJ, USA) for 10 min and centrifuged. ..

Article Title: Boosting the immune response in COVID-19 vaccines via an Alum:CpG complex adjuvant.
Article Snippet: Selecting appropriate adjuvants is crucial for developing an effective vaccine.. However, studies on the immune responses triggered by different adjuvants in COVID-19 inactivated vaccines are scarce.. Herein, we evaluated the efficacy of Alum, CpG HP021, Alum combined with CpG HP021 (Alum/CpG), or MF-59 adjuvants with COVID19 inactivated vaccines in K18-hACE2 mice, and compared the different immune responses between K18-hACE2 and BALB/c mice.

Article Title: Homologous but not heterologous COVID-19 vaccine booster elicits IgG4+ B-cells and enhanced Omicron subvariant binding.
Article Snippet: Briefly, 10–15million PBMC were incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD21, CD27, CD38, CD71, IgA, IgD, IgG1, IgG2, IgG3, IgG4, (Cat numbers and dilutions provided in Supplementary Tables 4 and 5) and 5 μg/ml each of [RBDWH1]4-BUV395, [RBDWH1]4BUV737, and [RBD BA.2]4-BV480 and [RBD BA.5]4-BV650 for 15min at room temperature in a total volume of 250 μl FACS buffer (0.1% sodium azide, 0.2% BSA in PBS). .. In addition, 5 million PBMC were similarly incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD27 and IgD, and BUV395-, BUV737-, BV480- and BV650-conjugated streptavidin controls (Supplementary Tables 4 and 5). ..

Incubation:

Article Title: Homologous but not heterologous COVID-19 vaccine booster elicits IgG4+ B-cells and enhanced Omicron subvariant binding
Article Snippet: .. Briefly, 10–15 million PBMC were incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD21, CD27, CD38, CD71, IgA, IgD, IgG1, IgG2, IgG3, IgG4, (Cat numbers and dilutions provided in Supplementary Tables and ) and μg/ml each of [RBD WH1] -BUV395, [RBD WH1] -BUV737, and [RBD BA.2] -BV480 and [RBD BA.5] -BV650 for 15 min at room temperature in a total volume of 250 μl FACS buffer (0.1% sodium azide, 0.2% BSA in PBS). .. In addition, 5 million PBMC were similarly incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD27 and IgD, and BUV395-, BUV737-, BV480- and BV650-conjugated streptavidin controls (Supplementary Tables and ).

Article Title: Homologous but not heterologous COVID-19 vaccine booster elicits IgG4+ B-cells and enhanced Omicron subvariant binding
Article Snippet: Briefly, 10–15 million PBMC were incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD21, CD27, CD38, CD71, IgA, IgD, IgG1, IgG2, IgG3, IgG4, (Cat numbers and dilutions provided in Supplementary Tables and ) and μg/ml each of [RBD WH1] -BUV395, [RBD WH1] -BUV737, and [RBD BA.2] -BV480 and [RBD BA.5] -BV650 for 15 min at room temperature in a total volume of 250 μl FACS buffer (0.1% sodium azide, 0.2% BSA in PBS). .. In addition, 5 million PBMC were similarly incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD27 and IgD, and BUV395-, BUV737-, BV480- and BV650-conjugated streptavidin controls (Supplementary Tables and ). ..

Article Title: Homologous but not heterologous COVID-19 vaccine booster elicits IgG4+ B-cells and enhanced Omicron subvariant binding.
Article Snippet: Briefly, 10–15million PBMC were incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD21, CD27, CD38, CD71, IgA, IgD, IgG1, IgG2, IgG3, IgG4, (Cat numbers and dilutions provided in Supplementary Tables 4 and 5) and 5 μg/ml each of [RBDWH1]4-BUV395, [RBDWH1]4BUV737, and [RBD BA.2]4-BV480 and [RBD BA.5]4-BV650 for 15min at room temperature in a total volume of 250 μl FACS buffer (0.1% sodium azide, 0.2% BSA in PBS). .. In addition, 5 million PBMC were similarly incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD27 and IgD, and BUV395-, BUV737-, BV480- and BV650-conjugated streptavidin controls (Supplementary Tables 4 and 5). ..

FACS:

Article Title: Homologous but not heterologous COVID-19 vaccine booster elicits IgG4+ B-cells and enhanced Omicron subvariant binding
Article Snippet: .. Briefly, 10–15 million PBMC were incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD21, CD27, CD38, CD71, IgA, IgD, IgG1, IgG2, IgG3, IgG4, (Cat numbers and dilutions provided in Supplementary Tables and ) and μg/ml each of [RBD WH1] -BUV395, [RBD WH1] -BUV737, and [RBD BA.2] -BV480 and [RBD BA.5] -BV650 for 15 min at room temperature in a total volume of 250 μl FACS buffer (0.1% sodium azide, 0.2% BSA in PBS). .. In addition, 5 million PBMC were similarly incubated with fixable viability stain 700 (BD Biosciences), antibodies against CD3, CD19, CD27 and IgD, and BUV395-, BUV737-, BV480- and BV650-conjugated streptavidin controls (Supplementary Tables and ).

Labeling:

Article Title: Boosting the immune response in COVID-19 vaccines via an Alum:CpG complex adjuvant.
Article Snippet: Selecting appropriate adjuvants is crucial for developing an effective vaccine.. However, studies on the immune responses triggered by different adjuvants in COVID-19 inactivated vaccines are scarce.. Herein, we evaluated the efficacy of Alum, CpG HP021, Alum combined with CpG HP021 (Alum/CpG), or MF-59 adjuvants with COVID19 inactivated vaccines in K18-hACE2 mice, and compared the different immune responses between K18-hACE2 and BALB/c mice.



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